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Journal: Cell Death & Disease
Article Title: ROR2 suppresses metastasis of prostate cancer via regulation of miR-199a-5p–PIAS3–AKT2 signaling axis
doi: 10.1038/s41419-020-2587-9
Figure Lengend Snippet: ( a ) PC-3 human PCa cells (1 × 10 6 ) were directly injected into prostate organ of nude mice to form prostate tumors. There were five mice in control group and experimental group, respectively. Protein expression level of ROR2, PIAS3, E-cadherin, and vimentin was examined by IHC. Images were captured at ×40 magnification. Scale bar represented 50 μm. ( b ) Proteins level of ROR2, PIAS3, phospho-AKT S473, phospho-AKT T308, and total AKT in prostate xenografts were also determined by the western blotting assay. Expression of β-actin was used as loading control. ( c ) Hematoxylin and eosin staining of lung tissues in mice was examined for dissemination of PCa cells. Images were capture at ×20 (upper panel) and ×40 (bottom panel) magnification. Scale bar represented 50 μm. ( d ) A list of lung metastasis of PC-3 cells with or without ROR2 overexpression in the above orthotopic nude mice.
Article Snippet: Paraffin embedded tissue sections were derived from commercial
Techniques: Injection, Expressing, Western Blot, Staining, Over Expression